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Evidence Library

Three trials. Three species. One platform.

Peer-reviewed in vivo validation of the E. coli + Salmonella phage backbone across broilers, layers, and Ma-chicken. Plus: Komipharm in vitro aquaculture validation.

4
Studies on file
4,970
Animals in vivo
3
Peer-reviewed journals
−7.4%
FCR improvement (best case)
Evidence classification: Published study Peer-reviewed in vivo trial Validated In Vitro In vitro lytic assay only Platform-Supported Backbone data, no standalone trial
In Vivo Trials

Poultry in vivo — E. coli + Salmonella backbone.

Three peer-reviewed trials from Ge L. et al. (Feed Research, 2020–2022). Authorized English translations on file. All trials use the PHAGIX FORTE P E. coli + Salmonella phage actives.

Trial 1 — White-Feather Broilers

Linyi, Shandong, China · 42 days · n=1,500 day-old chicks · 200 g/MT FORTE P
Published study
Ge L. et al.
Feed Research 2020 (04)
Design. 5 treatments × 10 replicates × 30 birds. Control vs antibiotic (Nosihepde 300 g/t + Chlortetracycline 200 g/t) vs PHAGIX™ FORTE P at 100, 200, and 500 g/t. Endpoints: growth performance, diarrhea, mortality, cecal microflora, immune organ indices, serum IgA/IgG/IgM.
Parameter Control Antibiotic PHAGIX™ FORTE P 200 g/t
F/G (feed-to-gain)1.631.521.51 · −7.36%
ADG (g/bird·d)65.3771.8572.39 · +5.31%
Diarrhea rate (%)18.676.335.67
Mortality rate (%)9.675.334.33 · −55%
E. coli (log CFU/g cecum)6.776.015.38 · −1.39 log
Salmonella (log CFU/g cecum)4.874.663.38 · −1.49 log
Lactic acid bacteria (log CFU/g)7.526.638.23 · +0.71 log ↑
Serum IgG (g/L)6.726.657.56

DOI: 10.13557/j.cnki.issn1002-2813.2020.04.029 · Authorized translation on file · Note: PHAGIX increased beneficial Lactobacillus (+0.71 log); antibiotic suppressed it (6.63 vs 7.52 control).

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Trial 2 — Hy-Line Brown Layers

Handan, Hebei, China · 30 days · n=2,400 layers (50 weeks) · 300 g/MT FORTE P
Published study
Ge L. et al.
Feed Research 2020 (07)
Design. 4 treatments × 6 replicates × 100 birds. Control vs PHAGIX™ FORTE P at 100, 300, and 500 g/t. Endpoints: laying rate, egg weight, FCR, defective egg rate, Haugh unit, shell quality, intestinal microflora.
Parameter Control PHAGIX™ FORTE P 300 g/t Δ vs control
Laying rate (%)90.3592.56+2.45%
FCR (feed:egg)2.222.11−4.95%
Defective egg rate (%)2.511.07−57.37%
Haugh unit86.7690.19+3.95%
E. coli (log CFU/g cecum)6.875.51−1.36 log
Salmonella (log CFU/g cecum)4.773.55−1.22 log

DOI: 10.13557/j.cnki.issn1002-2813.2020.07.031 · Authorized translation on file · Note: Defective egg rate halved — direct downstream revenue impact for layer programs.

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Trial 3 — Guangxi Ma-Chicken

Guangxi, China · 35 days · n=1,050 Ma-chicken · 100 g/MT PHAGIX + 100 g/MT AMP
Published study
Ge L. et al.
Feed Research 2022 (07)
Design. 7 treatments × 5 replicates × 30 birds. Combinations of PHAGIX™ FORTE P (50, 100, 150, 300 mg/kg) with antimicrobial peptide (AMP). Endpoints: ADG, F/G, diarrhea, mortality, gut microflora, immune organs.
Treatment group F/G Diarrhea (%) Mortality (%) Outcome
Control2.629.338.67Baseline
PHAGIX™ alone · 300 mg/kg2.514.005.33−4.20% F/G
PHAGIX™ + AMP · 300/300 mg/kg2.493.334.00−4.96% F/G · lowest mortality
PHAGIX™ + AMP · 100/100 mg/kg2.524.674.67Recommended — best cost-efficacy

DOI: 10.13557/j.cnki.issn1002-2813.2022.07.024 · Authorized translation on file · Note: IgG increased +49% in combination groups — phage + AMP synergy enhances immune response alongside pathogen suppression.

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In Vitro Validation

Aquaculture phage activity — Komipharm International.

Aquaculture Lytic Assay — Seven PHAGIX Isolates

Komipharm International Co., Ltd. · Siheung, South Korea · In vitro assay
Validated In Vitro
Komipharm International
Siheung, South Korea
Scope. Seven PHAGIX phage isolates tested at 2×10⁷ PFU/mL challenge. Target strains: V. scophthalmi, V. harveyi, A. salmonicida masoucida, S. parauberis. Dilution series tested through 10⁹ dilution.
MetricResult
Isolates producing confluent lysis7/7 (100%)
Challenge concentration2×10⁷ PFU/mL
Dilution rangeThrough 10⁹ dilution
Target strains confirmedV. scophthalmi · V. harveyi · A. salmonicida · S. parauberis
⚑ Scope of this validation

The Komipharm assay confirms in vitro lytic potency and host specificity. This is not a field trial. No in vivo aquaculture trials are on file for PHAGIX VIBRA FORTE. In vivo aquaculture trial data is being accumulated through ongoing commercial programs. The platform argument: same host-specific lytic technology validated in vivo in poultry, validated in vitro against aquaculture pathogens. Contact KYNAD for the current program status.

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Authorized English translations of all three peer-reviewed Feed Research studies, the Komipharm in vitro assay, and the Clostridium bacteriophage poultry trial dossier. Available to technical, regulatory, and integrator teams on request.