Precision Nutrition · Vitamin D₃ technology

D-VITIX™ D₃ — Nature's Boost.

Rapid absorption for stronger livestock.

A 25-Hydroxyvitamin D₃ (Calcifediol) feed additive that bypasses hepatic hydroxylation — delivering the active metabolite directly to where it's needed for calcium metabolism, skeletal integrity, immune resilience, and reproductive performance.

25-Hydroxyvitamin D₃ · also known as Calcifediol or 25(OH)D₃
CAS No. 19356-17-3
Active concentrations 1.25% · 0.05%
Form Microencapsulated powder
Liquid customizable
Thermal stability Stable to 90 °C
Pellet-feed compatible
Shelf life 24 months
Cool, dry, sealed

What It Is — and why it works faster.

Conventional Vitamin D₃ (cholecalciferol) requires hepatic conversion to 25-Hydroxyvitamin D₃ before it can act on calcium and phosphorus metabolism. D-VITIX™ delivers the active metabolite directly — bypassing that liver-dependent step entirely.

D-VITIX™ D₃ is 25-Hydroxyvitamin D₃ (Calcifediol). Unlike cholecalciferol, it bypasses the liver's first hydroxylation step and rapidly elevates serum 25(OH)D₃ levels — within 48–72 hours in feeding studies. The result is faster physiological impact on calcium and phosphorus regulation, immune cell signaling, and reproductive performance.

Standard reference

Cholecalciferol (Vitamin D₃)

  • AbsorptionIntestine + liver-dependent activation
  • BioactivityDelayed — requires hepatic hydroxylation
  • Circulating 25(OH)D₃Low
  • Bone impactModerate
  • Immune supportLimited
  • Feed processingLess stable
D-VITIX™ D₃

25-Hydroxyvitamin D₃ (Calcifediol)

  • AbsorptionDirect intestinal — no liver step
  • BioactivityImmediate · ≈3× faster activation
  • Circulating 25(OH)D₃High — elevated within 48–72 h
  • Bone impactSuperior — measured bone ash & density gains
  • Immune supportEnhanced — direct immune cell signaling
  • Feed processingStable to 90 °C (microencapsulated beadlet)

Dosing Matrix — two concentrations, one chemistry.

D-VITIX™ ships in two active concentrations. 1.25% is the high-efficiency choice for low-inclusion premixes — typical for integrators optimizing formulation efficiency. 0.05% offers higher-inclusion flexibility for commercial feed mills and pre-starters.

1.25%
25-Hydroxyvitamin D₃ · Min.

D-VITIX™ D₃ 1.25

High-efficiency inclusion for low-dosage premixes. Preferred by integrators seeking formulation efficiency in vitamin/mineral premixes and concentrated feed blends.

Poultry4 g/MT feed
Swine5 g/MT feed
0.05%
25-Hydroxyvitamin D₃ · Min.

D-VITIX™ D₃ 0.05

Higher-inclusion flexibility for feed manufacturers. Easy to dose across compound feed, pre-starters, and customizable premix formulas. Suited to commercial mills and specialty feeds.

Poultry100 g/MT feed
Swine125 g/MT feed

Best results achieved under well-balanced nutrition programs with adequate calcium and phosphorus levels. KYNAD technical team provides customized dosage recommendations based on livestock species, production goals, and feed format.

Field Trial Data — broilers, layers, breeders, swine.

Performance data from controlled trials across multiple research institutions and commercial operations. Every figure shown is from a specific study — institution and design noted on each panel.

Broiler trial · performance & bone health

Southern Poultry Research Group (USA) 1,500 Ross × Ross 708 male broilers · 43 days
Design. 4 treatments × 15 replicate pens × 25 birds. Three-phase feeding (Starter D0–14, Grower D14–28, Finisher D28–43). Inclusion: 25(OH)D₃ at 69 ppb (D-VITIX, treatment A) or 34.5 ppb (treatment B); basal Vitamin D₃ at 5.5 ppm across all treatments to prevent osteochondrosis. Zoalene added for coccidiosis control. Whole right tibia & plasma collected D21 & D43.
Parameter Control Vitamin D₃ D-VITIX 25(OH)D₃
Body Weight Gain (g/bird) 1,940 2,005 2,055
Feed Conversion Ratio (FCR) 1.68 1.63 1.60
Tibial Ash (%) 42.1 44.8 47.0
Serum 25(OH)D₃ — Day 43 D-VITIX group significantly elevated vs. control & Vitamin D₃ groups Highest

Cross-trial summary including layer outcomes: eggshell thickness 0.39 mm (vs control 0.34); egg production 91.8% (vs control 86.5%).

Layer trial · production rate & shell quality

Yichun layer farm, Jiangxi (China) Hy-Line Brown laying hens · 430–465 days
Design. Control group: basal diet. Experimental group: basal diet + 62.5 μg/kg 25(OH)D₃ (D-VITIX). Outcomes measured over commercial production cycle.
Parameter Control D-VITIX 25(OH)D₃ Δ
Production Rate (%) 83.04 85.36 +2.79%
Egg Breaking Rate (%) 1.86 1.39 −25.27%
Feed to Egg Ratio 2.254 2.171 −3.68%
Egg Weight (g) 61.53 61.89 +0.73%

Breeder trial · fertility & hatchability

Qingyuan Ma breed (China) Breeder hens at 4 laying weeks · 16-week test period
Design. Control: basal diet. Experimental: basal diet + 62.5 μg/kg 25(OH)D₃ (D-VITIX).
Parameter Control D-VITIX 25(OH)D₃ Δ
Rate of genuine eggs (%) 72.93 73.83 +1.23%
Hatching rate (%) 89.47 90.48 +1.13%
Individual chicken hatching rate (%) 65.25 66.17 +1.41%

Swine trial · piglet growth & immunity

Hunan Animal Science Institute (China) Piglets & weaners
Design. Piglets fed 25(OH)D₃ (D-VITIX) vs. cholecalciferol vs. control. Endpoints: ADG, FCR, pre-weaning mortality, immune markers, weaning weight.
Parameter Control Vitamin D₃ D-VITIX 25(OH)D₃
Average Daily Gain (g/day) 490 510 540
FCR 2.63 2.58 2.51
Pre-weaning Mortality (%) 12.4 10.2 8.8
IgG levels (mg/dL) 920 980 1,050
Weaning Weight (kg) 6.2 6.7 7.1

Trial outcomes shown as published. Actual outcomes vary by operation, baseline performance, feed program, and environmental conditions. Additional study sources (Southern China Poultry Institute breeder work, INRA France longevity data, University of Wisconsin calf trials, New Hope Feed Research Institute layer trials) available in the full D-VITIX Introduction Book on request.

Application by species.

D-VITIX™ D₃ is applied across poultry and swine programs. Ruminant applications are emerging — strong scientific foundation, with early adopters reporting consistent performance gains in calves and dairy.

Poultry

Established · broilers, layers, breeders
  • Improved bone strength and walking ability in broilers
  • Enhanced shell thickness and egg quality in layers
  • Improved feed efficiency and flock uniformity
  • Reduced skeletal disorders (tibial dyschondroplasia, rickets)
  • Higher fertility and hatchability in breeder lines

Swine

Established · sows, weaners, growers, finishers
  • Supports sow bone mineralization & lactation
  • Enhances piglet viability & early growth
  • Improves ADG, FCR, carcass yield in growers
  • Reduces pre-weaning mortality & improves uniformity
  • Stronger response to E. coli & Mycoplasma challenges

Ruminants

Emerging · calves, dairy, beef
  • Supports mineral metabolism in calves & dairy
  • Enhances bone density, reduces rickets risk in young ruminants
  • Improves plasma calcium under heat stress (dairy)
  • Faster weight gain & lower diarrhea in calves
  • Reduced hoof issues in intensive-finishing bulls (pilot)

Specifications.

Complete product specifications for D-VITIX™ D₃ 1.25 and D-VITIX™ D₃ 0.05. Certificate of analysis and SDS available on request.

Parameter D-VITIX™ D₃ 1.25 D-VITIX™ D₃ 0.05
Active ingredient25-Hydroxyvitamin D₃ — Min. 1.25%25-Hydroxyvitamin D₃ — Min. 0.05%
CAS No.19356-17-3 (Calcifediol)19356-17-3 (Calcifediol)
Other ingredientsMaltodextrin, plant oil, antioxidantMaltodextrin, plant oil, antioxidant
AppearanceUniform white powder, good flowabilityUniform white powder, good flowability
Particle size>95% through 1.18 mm sieve>95% through 1.18 mm sieve
Moisture contentMax. 5%Max. 5%
SolubilityDispersible in waterDispersible in water
Thermal stabilityUp to 90 °C (pellet-feed compatible)Up to 90 °C (pellet-feed compatible)
Shelf life24 months24 months
Package20 kg cartons · sealed bags
Bulk options on request
20 kg cartons · sealed bags
Bulk options on request
Hazard classificationNon-hazardous · EC No. 1272/2008Non-hazardous · EC No. 1272/2008

Quality & analytical method.

D-VITIX™ is manufactured under ISO 9001 and FAMI-QS quality systems, with GMP-compliant production. Analytical release testing uses a validated reversed-phase HPLC/UPLC method with UV detection at 265 nm — full SOP available to QA teams on request.

Quality systems Manufactured under ISO 9001 · FAMI-QSGMP-compliant production
Test method Reversed-phase HPLC/UPLCUV detection · 265 nm
Linearity r² ≥ 0.999Range 5–80 μg/mL
Accuracy 97.8–102.1%Spike recovery
Precision %RSD ≤ 2%Inter-day ≤ 3%

Storage & handling.

D-VITIX™ is sensitive to light, heat, and humidity. Store sealed in original packaging; once opened, use quickly. Standard PPE applies during handling.

Storage

  • Dry, cool, well-ventilated Avoid direct sunlight, high humidity, and excessive heat. Original sealed packaging preserves activity through full 24-month shelf life.
  • Light-sensitive Vitamin D₃ metabolites are photodegradable. Keep packaging closed when not dispensing; minimize exposure to natural and UV light sources.
  • Use quickly once opened Once seal is broken, schedule use to minimize ambient exposure. Microencapsulated beadlet protects against routine premix conditions.

Handling

  • PPE Safety goggles recommended. Handle in a well-ventilated area. Standard feed-additive handling practices apply.
  • Hazard classification Per EC No. 1272/2008 (and amendments): non-hazardous. No hazard pictograms, warning words, or precautionary advice required.
  • Environmental Non-hazardous to environment under normal use. No foreseeable environmental threat under standard handling and disposal conditions.
Request

Request the full D-VITIX™ packet.

Technical brief, specification sheets for both concentrations, certificate of analysis, analytical method SOP, and the trial dossier (35-slide Introduction Book including additional layer, breeder, ruminant, and field application data).